Preparation steps before buccal swab DNA testing
Collecting DNA samples from the buccal swab is a convenient, safe and painless method, suitable for genetic testing such as paternity testing. It can be tested on all genders and all ages, starting from newborns. In this article, we will explain the steps to prepare for a DNA test at HealthSmile in detail to get the most accurate results.
1. Equipment used for DNA testing from buccal mucosa.
- Sterile Buccal Swab: The swab is made of plastic and the tip is sterile polyester. HealthSmile uses DNA swabs from the United States, brand Puritan®, for DNA Paternity test (Non-legal).
- Clean and dry sample collection bags: 1 bag per person
- Gloves to protect against infection and secretions (if available)
- Plastic envelopes / shockproof parcel envelopes for storing samples for delivery via various transport methods
- Form for filling in information of the examinee (you can read how to fill in information in this article)
2. Preparation steps before buccal swab DNA examination
- Avoid eating, drinking, smoking, or chewing gum for at least 30 minutes prior to sample collection.
- Prepare identification documents such as your national ID card or passport.
- Wash hands thoroughly or wear gloves (if available) to reduce the chance of contamination from external DNA.
3. DNA sample collection steps
- Open the sample collection stick
- Carefully unpack the sample container.
- Hold the sample collection stick by the stem, avoiding touching the polyester tip to prevent contamination.
- Samples were collected from the buccal mucosa.
- Use a swab to swab the inside of your mouth from your cheek.
- Rub back and forth for about 10-15 seconds, applying slight pressure to capture enough cells.
- Repeat on the opposite side using a new sampling stick.
- Dry the sample
- Place the sample stick in a clean, dry place and let it air dry for about 5-10 minutes.
- Avoid blowing on or using heat as this can damage DNA.
- Place the sample in the prepared container.
- When completely dry, place the sample collection stick into the prepared envelope.
- Keep containers tightly closed to prevent environmental contamination.
- Label and store
- Write your name, date, and relevant information on the label.
- Store samples at room temperature and avoid direct sunlight.
- If it needs to be sent to a laboratory, it should be delivered as soon as possible within 24-48 hours.
4. Precautions for collecting DNA samples
- Do not use the same swab for multiple people.
- Avoid touching the tip of the DNA collection rod with bare hands.
- Do not allow the sample to get wet or exposed to chemicals, as this may cause DNA degradation.
- If the sample is collected for forensic purposes or for use in legal proceedings, there should be witnesses to verify the information and proper data recording methods should be used.
5. Summary
Collecting DNA samples from the buccal mucosa is a simple and convenient method. If the steps are followed correctly, it will help to obtain high-quality samples, reduce the chances of contamination, and make the test results more accurate. If you want to use the DNA testing service, you should choose a certified and standardized testing center, such as HealthSmile , which offers parent-child testing and genetic disease testing using modern technology.
If you have any further questions about DNA sample collection, you can contact our experts for further advice at LINE ID: @HealthSmile.
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